BioWire Daily Rotations

From FreeBio

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Daily Rotations

Day One (Danny & Orr)
  • Pick colonies into cultures

Day Two (Sasha & Kang-Xing)

  • Freezer stock
  • Miniprep (check for pSB2K3 and use IPTG)
  • Analytical Digest
  • Digest (prefix/suffix, vector/insert)
  • Make gel

Day Three (Connie & Patrick)

  • CIP the vector
  • Run gel
  • Gel extraction
  • Ligation
  • Transformation
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